학술논문
Phase determination of the Fructose-6-phosphate aldolase 1 crystal from Aeromonas media
이용수 4
- 영문명
- Phase determination of the Fructose-6-phosphate aldolase 1 crystal from Aeromonas media
- 발행기관
- 한국구조생물학회
- 저자명
- Pil-Won Seo Jeong-Sun Kim
- 간행물 정보
- 『Biodesign』Vol 10, No 4, Dec, 64~68쪽, 전체 5쪽
- 주제분류
- 자연과학 > 생물학
- 파일형태
- 발행일자
- 2022.12.31
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국문 초록
영문 초록
Fructose-6-phosphate aldolase 1 (FSA) from Aeromonas media (AmFSA) is a class I aldolase characterized by the formation of a Schiff-base intermediate between a conserved lysine residue and a carbonyl substrate. FSA has shown high stereoselectivity and broad substrate spectrum. To provide a structural background for engineering as a catalyst for desired reaction product, AmFSA was cloned and expressed. The purified protein was crystallized from the precipitant of 0.1 M sodium acetate (pH 5.0), 1.4 M ammonium sulfate, and 10 mM β-mercaptoethanol. Diffraction data were collected to 2.1 Å resolution. The crystal belonged to the orthorhombic space group C2221 with unit-cell parameters a = 93.80 Å, b = 175.27 Å, c = 150.99 Å, and α = β = γ = 90°. The spatial positions of the five protein molecules in the asymmetric unit were determined by molecular replacement using the sequentially related Escherichia coli FSA structure.
목차
INTRODUCTION
RESULTS AND DISCUSSION
METHODS
REFERENCES
키워드
해당간행물 수록 논문
- Phase determination of the Fructose-6-phosphate aldolase 1 crystal from Aeromonas media
- Structural and biochemical analysis of Glyceraldehyde-3-Phosphate Dehydrogenase from Clostridium beijerinckii
- Crystallization and preliminary diffraction analysis of the T2I·L262F double mutant form of SHP2
- Yeast nicotinate-nucleotide pyrophosphorylase in complex with ligand: crystallization and preliminary structural approaches
- Cryo-Electron Microscopy Research Center at Pusan National University
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