Crystal structure of cobolimab, an investigational antibody against Tim-3 for cancer immunotherapy, in complex with anti-kappa nanobody

• 영문명: Crystal structure of cobolimab, an investigational antibody against Tim-3 for cancer immunotherapy, in complex with anti-kappa nanobody
• 발행기관: 한국구조생물학회
• 저자명: Seung Beom Choi Nahyeon Gu Yu-Jeong Jang Ui Beom Park Tae Jun Jeong Hyun Tae Lee Yong-Seok Heo
• 간행물 정보: 『Biodesign』Vol 10, No 3, Sep, 46~50쪽, 전체 5쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2022.09.30

영문 초록

Cobolimab is an investigational antibody targeting Tim-3, an immune checkpoint protein associated with the suppression of T-cell response against cancer cells. This antibody’s safety and anti-tumor effectiveness were made clear in the clinical trial of the combination therapy using cobolimab and a PD-1 antibody. Here, the crystal structure of the cobolimab Fab in complex with an anti-kappa nanobody is determined. The crystals belonged to the space group C2221, with unit cell parameters a = 64.30, b = 119.30, and c = 184.49 Å. The structure was refined to a resolution of 2.02 Å with Rwork/Rfree = 0.197/0.233. The nanobody in the structure facilitated crystallization by restricting the elbow flexiblity of the Fab and providing extra packing interface for crystallization. This structure can be used as a model structure for predicting the protein-protein interaction between cobolimab and Tim-3.