나노기둥구조 크기가 섬유아세포의 증식 및 alpha-smooth muscle actin (α-SMA)의 발현에 미치는 영향

• 영문명: The influence of nanopillar structure size to fibroblast’s proliferation and alpha-smooth muscle actin (α-SMA) expression
• 발행기관: 대한두개저학회
• 저자명: 강은혜(Eun Hye Kang) 이상수(Sang Soo Lee) 윤영식(Young Sik Yun) 이원재(Won Jai Lee) 여종석(Jong Souk Yeo) 노태석(Tai Suk Roh) 윤인식(In Sik Yun)
• 간행물 정보: 『대한두개저학회지』제15권 제2호, 98~104쪽, 전체 7쪽
• 주제분류: 의약학 > 일반외과학
• 파일형태: PDF
• 발행일자: 2020.10.30

영문 초록

Backgound : With advances in medicine and materials engineering, biomaterials, such as silicone and titanium are used in many tissue regeneration cases. Many studies are being conducted to confirm the reactivity of cells to various biomaterials. Among these studies, fibroblast proliferation and differentiation are known as important factors related to capsulation. In this study, we attempted to determine how various sized polyurethane acrylate (PUA) nanopillar structures act on fibroblast proliferation and differentiation. Methods : The scaffolds were prepared in four groups: flat and nanopillar (350 nm, 500 nm, 1,000 nm). Flat group was used as a control. To evaluate the effect of scaffolds of nanopillar structures on fibroblasts, quantitatively analyzed for the degree of adhesion, proliferation and alpha-smooth muscle actin (α-SMA) expression of cells. Results : As a result of the MTT assay, the proliferation was lower than that of the flat group in the nanopillar group. As a result of staining, the intensity of F-actin was significantly different from that of the flat group. And the intensity of α-SMA was different from that of the flat group but not significantly. Conclusions : The nanopillar structure of PUA material seems to play a role in inhibiting the proliferation and differentiation of fibroblasts regardless of the size of the structure. This suggests that the use of PUA nanopillar implants may be effective in suppressing fibrosis due to fibroblast proliferation and differentiation.

목차

INTRODUCTION

METHODS

RESULTS

DISCUSSION

키워드

Nanopillar Fibroblast Cell attachment Cell proliferation