The Effect of Low Frequency Electromagnetic Fields on the Proliferation of vestibular schwann cells

• 발행기관: 대한두개저학회
• 저자명: Yeo Jun Yoon In Seok Moon Gwan Soo Park Jong Dae Lee Kiwon Song
• 간행물 정보: 『대한두개저학회지』제11권 제2호, 53~58쪽, 전체 6쪽
• 주제분류: 의약학 > 일반외과학
• 파일형태: PDF
• 발행일자: 2016.09.30

영문 초록

Objectives : Authors reported that the time of mobile phone usage can be related with the vestibular schwannoma growth. In this study, we tried to approach to the biological effect of the extremely low frequency electromagnetic field (ELF-EMF) on NF2-/- mouse schwann (SC4) cells. Experimental Design : We used SC4 cell line and optimized the ELF-EMF exposure conditions with the ELF-EMF exposure results of other cells. For acute response, we exposed 1 and 10mT ELF-EMF to SC4 cells for 30minutes. For continuous exposures, SC4 cell were incubated in the 1 and 10mT ELF-EMF up to 144 hr. Cell viability was measured by MTT assays and proteins were detected by western blots. Distribution of cells in each stage of the cell cycle was analyzed by Fluorescence-activated cell sorting (FACS). Results : SC4 cells exposed to the continuous as well as the acute ELF-EMF did not show any DNA double strand breaks and apoptosis up to 144 hr. Interestingly, the proliferation of SC4 cells was increased by continuous exposures to ELF-EMF in an intensity and dose dependent manner. However, no activation of Erk and Akt pathways was observed in these cells. Moreover, both the EMF-exposed and unexposed SC4 cells showed similar distribution of cells in each stage of the cell cycle. Conclusion : These results demonstrated that ELF-EMF of the similar total value of multiplied energy and exposure time of RF-EMF can accelerate the proliferation of SC4, indirectly supporting a positive correlation between the cell phone usage and the tumor size.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

키워드

vestibular schwannoma mobile phone extremely low frequency electromagnetic field (ELF-EMF) NF2 -/- mouse schwann (SC4) cells