Development of Molecular Markers for Low Raffinose and Stachyose in Korean Soybean Cultivars

• 발행기관: 한국육종학회
• 저자명: Kiwoung Yang Jong-Min Ko Tae Joung Ha Yeong-Hoon Lee In-Youl Baek Tae-Jin Yang Ill-Sup Nou
• 간행물 정보: 『Plant breeding and biotechnology』Vol.2 No.2, 151~157쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2014.06.01

영문 초록

A novel allele of the putative soybean raffinose synthase gene, RS2, was discovered in PI200508 that is associated with the low raffinose and stachyose content. Soybean line PI200508 was identified as expressing reduced levels of raffinose and stachyose as well as elevated levels of sucrose. The RS2 mutant gene shows three base pairs InDel with the normal gene. Based on InDel region we developed novel co-dominant and dominant marker. The aim of this study was to develop Korean soybean cultivars, Daewon, Cheongja, and Danmiput, containing low levels of raffinose and stachyose. A specific markers assay for the PI200508 RS2 allele was developed to allow direct selection of the low raffinose and stachyose phenotype. Our findings highlight the efficiency of allele-specific markers in selection, which is evident in the matching genotype and results of the HPLC in the F2 generations of Daewon×PI200508 population.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGMENT

REFERENCES

키워드

HPLC analysis Korean soybean Low raffinose and stachyose Molecular marker SNP and InDel