Identification of a New Race and Development of DNA Markers Associated with Powdery Mildew in Melon

• 발행기관: 한국육종학회
• 저자명: Hoy-Taek Kim Jong-In Park Arif Hasan Khan Robin Tomoko Ishikawa Maki Kuzuya Manabu Horii Katsutoshi Yashiro Ill-Sup Nou
• 간행물 정보: 『Plant breeding and biotechnology』Vol.4 No.2, 225~233쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.06.01

영문 초록

Powdery mildew disease caused by an obligatory parasitic fungus Podosphaera xanthii is a serious problem of melon (Cucumis melo L.) production worldwide. Severity of problem is further associated with emergence of new races over the years. In this study a new race of powdery mildew fungus was discovered from Ibaraki, Japan. The race was different from all other existing races of P. xanthii occurring in Japan. Phenotypic and genetic analysis established the new fungus type as a new race, N5. Ten melon lines were infected with a total of eight fungal races including the new N5 race and it was found that all melon lines had different disease reactions against the new race compared to other seven races. Only four melon genotypes were found resistant out of 42 commercial cultivars and lines were tested. Disease reactions of two sets of F2 populations and one set of backcross population revealed that two separate epistatic gene loci located in two different linkage groups (LG), LG II and LG XII, interact together for the resistant or susceptible reaction of melon lines. A total of six simple sequence repeat (SSR) markers were found polymorphic in melon lines out of 16 tested in response to N5 race. Two different sets of F2 populations between resistant and susceptible melon lines were assessed with two polymorphic SSR markers located in two different groups, LG II and LG XII. SSR genotyping yielded 78% and 94% expected polymerase chain reaction fragments in favor of resistance or susceptibility of F2 populations of CM17187×PMR5 and PMR45×PMR5 of melon lines, respectively.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Powdery mildew Melon Podosphaera xanthii Simple sequence repeat markers Genetic analysis