학술논문
Discrimination and Authentication of Eclipta prostrata and E. alba Based on the Complete Chloroplast Genomes
이용수 7
- 영문명
- 발행기관
- 한국육종학회
- 저자명
- Inseo Kim Jee Young Park Yun Sun Lee Hyun Oh Lee Hyun-Seung Park Murukarthick Jayakodi Nomar Espinosa Waminal Jung Hwa Kang Taek Joo Lee Sang Hyun Sung Kyu Yeob Kim Tae-Jin Yang
- 간행물 정보
- 『Plant breeding and biotechnology』Vol.5 No.4, 334~343쪽, 전체 10쪽
- 주제분류
- 농수해양 > 기타농수해양
- 파일형태
- 발행일자
- 2017.12.01
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국문 초록
영문 초록
Eclipta prostrata and E. alba are annual herbal medicinal plants and have been used as Chinese medicinal tonics. Both species are widely distributed in tropical and subtropical regions as well as in Korea. Both species have similar morphological features but E. alba has smoother leaf blade margins compared with E. prostrata. Although both species are utilized as oriental medicines, E. prostrata is more widely used than E. alba. Morphological semblances have confounded identification of either species. Here, we report the complete chloroplast genomes of both species to provide an authentication system between the two species and understand their diversity. Both chloroplast genomes were 151,733-151,757 bp long and composed of a large single copy (83,285-83,300 bp), a small single copy (18,283-18,346 bp), and a pair of inverted repeats (25,075-25,063 bp). Gene annotation revealed 80 protein coding genes, 30 tRNA genes and four rRNA genes. A phylogenetic analysis revealed that the genus Eclipta is grouped with Heliantheae tribe species in the Asteraceae family. A comparative analysis verified 29 InDels and 58 SNPs between chloroplast genomes of E. prostrata and E. alba. The low chloroplast genome sequence diversity indicates that both species are really close to each other and are not completely diverged yet. We developed six DNA markers that distinguish E. prostrata and E. alba based on the polymorphisms of chloroplast genomes between E. prostrata and E. alba. The chloroplast genome sequences and the molecular markers generated in this study will be useful for further research of Eclipta species and accurate classification of medicinal herbs.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
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