Mapping QTLs for Leafspot Resistance in Peanut Using SNP-Based Next-Generation Sequencing Markers

• 발행기관: 한국육종학회
• 저자명: Yuya Liang Michael Baring Shichen Wang Endang M. Septiningsih
• 간행물 정보: 『Plant breeding and biotechnology』Vol.5 No.2, 115~122쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.06.01

영문 초록

Leafspot is one of the major diseases of peanut (Arachis hypogaea L.) that can cause more than 50% yield loss. The objective of this study was to identify and map quantitative trait loci (QTLs) for resistance to leafspot disease. An F2:6 recombinant inbred line (RIL) population, derived from a released cultivar Tamrun OL07 and a highly tolerant breeding line Tx964117, were used as a mapping population. A total of 90 RILs were planted for disease phenotyping in Yoakum, Texas in 2010 and 2012. A genetic map spanning the 20 linkage groups was developed using 1,211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRAD-seq). A total of six quantitative trait loci (QTLs) were identified, with LOD score values of 3.2-5.0 and phenotypic variance explained ranging from 11%-24%. Major QTLs identified in this study may be used as potential targets for peanut improvement to leafspot disease through molecular breeding.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Peanut (Arachis hypogaea L.) Groundnut Leafspot disease QTL mapping