The Genes Associated with Drought Tolerance by Multi-Layer Approach in Potato

• 발행기관: 한국육종학회
• 저자명: Chang-Kug Kim Jae-Hyeon Oh Jong-Kuk Na Chuloh Cho Kyung-Hwa Kim Go Eun Yu Dool-Yi Kim
• 간행물 정보: 『Plant breeding and biotechnology』Vol.7 No.4, 405~414쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.12.01

영문 초록

We have developed a multi-layer pipeline that uses transcriptomic data to identify candidate genes associated with complex pathways in plants. We used this multi-layer approach, incorporating microarray, RNA sequencing, polymerase chain reaction (PCR), and protein-protein interaction analyses, to identify 14 genes associated with drought tolerance in potato. We generated transgenic potato lines that over-express bZIP28, one of the genes selected by our pipeline, to confirm the involvement of that gene in drought tolerance. The protein interactions of the products of the screened genes were assessed using yeast two-hybrid and bimolecular fluorescence complementation analyses. Overall, this study demonstrated the utility of our multi-layer approach for deciphering complex biosynthetic pathways using transcriptomic data.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

ACKNOWLEDGEMENTS

REFERENCES

키워드

Multi-layer approach Potato drought RNA sequencing