MITE-AFLP를 이용한 자포니카 벼의 다양성 검정

• 영문명: Diversity Analysis of Japonica Rice using MITE-transposon Display
• 발행기관: 한국작물학회
• 저자명: 인상낙(Sang-Nag Ahn) 홍성미(Seongmi Hong) 오창식(Oh chang sik) 권수진(Soo-Jin Kwon) Susan R. Wessler
• 간행물 정보: 『한국작물학회지』제51권 제3호, 259~268쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2006.06.30

영문 초록

Miniature inverted transposable elements (MITEs) are abundant genomic components in plant including rice. MITE-transposon display (MITE-TD) is an Amplified Fragment Length Polymorphism (AFLP)-related technique based on MITE sequence. In this study, we used the MITE-AFLP for the analysis of diversity and relationship of the 114 japonica accessions. Of the several MITEs, the mPing family was applied to detect polymorphisms based on PCR amplification. The BfaI adaptor primer and the specific primer derived from mPing terminal inverted repeat (TIR) region were used to PCR amplification of 114 accessions. Nine primer pairs produced a total of 160 polymorphic bands. PIC values of the polymorphic bands generated by nine primer pairs ranged from 0.269 (BfaI + ACT) to 0.426 (BfaI + T). Each accession revealed a distinct fingerprint with two primer combinations, BfaI + G and BfaI + C. Cluster analysis using marker-based genetic similarity classifed 114 accessions into five groups. MITE-AFLP markers were genetically mapped using a population of 80 BILs (BC1F7) derived from a cross between the rice accessions, Milyang 23 and Hapcheonaengmi 3. Eight of the markers produced with the primer pair BfaI + 0 were mapped on chromosomes 1, 2, 4, 5, 7, and 9. Considering that one MITE-AFLP marker on chromosome 7 was tightly linked to the Rc gene, the MITE-AFLP markers will be useful for gene tagging and molecular cloning.

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키워드

miniature inverted transposable elements (MITE) amplified fragment length polymorphism (AFLP) transposon display (TD) mPing terminal inverted repeat (TIR)