신 바이오디젤 원료 작물인 Camelina의 cDNA library 제작 및 유전자 특성

• 영문명: Construction and Characterization of a cDNA Library from the Camelina sativa L. as an Alternative Oil-Seed Crop
• 발행기관: 한국작물학회
• 저자명: 박원(Won Park) 장영석(Young-Seok Jang) 안성주(Sung-Ju Ahn)
• 간행물 정보: 『한국작물학회지』제55권 제2호, 151~158쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

Camelina sativa L., known as popular names “gold-of-pleasure” or “false flax” is an alternative oilseed crop that can be grown under different climatic and soil conditions. Up to date, however, the genomic information of Camelina has not been studied in detail. Therefore, a cDNA library was constructed and characterized from young leaves. The constructed cDNA library incorporated of 1334 cDNA clones and the size of the insertion fragments average was 736 base pair. We generated a total of 1269 high-quality expressed sequence tags (ESTs) sequences. The result of cluster analysis of EST sequences showed that the number of unigene was 851. According to subsequent analysis, the 476 (55.9%) unigenes were highly homologous to known function genes and the other 375 (44.1%) unigenes were unknown. Remaining 63 (7.4%) unigenes had no homology with any other peptide in NCBI database, indicating that these seemed to be novel genes expressed in leaves of Camelina. The database-matched ESTs were further classified into 17 categories according to their functional annotation. The most abundant of categories were “protein with binding function or cofactor requirement (27%)”, “metabolism (11%)”, “subcellular localization (11%)”, “cellular transport, transport facilities and transport routes (7%)”, “energy (6%)”, “regula-tion of metabolism and protein function (6%)”. Our result in this study provides an overview of mRNA expression profile and a basal genetic information of Camelina as an oilseed crop.

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Camelina sativa L. biodisel expressed sequence tag false flax gold-of-pleasure oilseed crop cDNA library