율무 새싹 추출물의 Riboflavin과 Coixol의 동시 분석법 검증 및 항산화 활성

• 영문명: Validation of a Method and Evaluation of Antioxidant Activity for the Simultaneous Determination of Riboflavin and Coixol in Coix lacryma-jobi var. ma-yuen Stapf Sprouts
• 발행기관: 한국작물학회
• 저자명: 이지연(Ji Yeon Lee) 박정용(Jung Yong Park) 박춘근(Chun-Geon Park) 김동휘(Dong Hwi Kim) 지윤정(Yun-Jeong Ji) 최수지(Su Ji Choi) 오명원(MyeongWon Oh) 황호섭(Hosop Hwang) 이윤지(Yunji Lee) 정진태(Jintae Jeong) 이정훈(Jeong Hoon Lee) 서경혜(Kyung Hye Seo)
• 간행물 정보: 『한국작물학회지』제64권 제4호, 452~458쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 °C under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC50], 68.9 ± 4.1 g/mL; and ABTS, IC50, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.

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키워드

bioactive compounds Coix lacryma-jobi correlation analysis free radical scavenging standardization