옥수수 종실 및 속대의 Phytosterol 동정과 함량 변이

• 영문명: Identification and Quantification of Phytosterols in Maize Kernel and Cob
• 발행기관: 한국작물학회
• 저자명: 김선림(Sun-Lim Kim) 김미정(Mi-Jung Kim) 정건호(Gun-Ho Jung) 이유영(Yu-Young Lee) 손범영(Beom-Young Son) 김정태(Jung-Tae Kim) 이진석(Jin-Seok Lee) 배환희(Hwan-Hee Bae) 고영삼(Young-Sam Go) 김상곤(Sang-Gon Kim) 백성범(Seong-Bum Baek)
• 간행물 정보: 『한국작물학회지』제63권 제2호, 131~139쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.06.30

영문 초록

Unsaponifiables in the kernel and the cob of 7 maize varieties were analyzed by using thin-layer chromatography (TLC) and gas chromatography (GC) for the identification of phytosterols and their concentrations. The unsaponifiables of the kernel were clearly separated into band I (campesterol, stigmasterol, and β-sitosterol), band Ⅱ (Δ5-avenasterol), band Ⅲ (Δ7- stigmastenol), and band Ⅳ (Δ7-avenasterol). In the cob, on the other hand, three or more bands were separated in addition to bands. The GC analysis of unsaponifiables showed good separation of campesterol, stigmasterol and β-sitosterol, but the mixture of Δ7- avenasterol (retention time[RT] 22.846), Δ7-stigmastenol (RT 22.852), and Δ5-avenasterol (RT 22.862) showed poor separation. Phytosterol content of the maize kernel was 635.9 mg/100 g, and that of the cob was 273.0 mg/100 g, respectively. The phytosterol content of the kernel was 2.4-fold higher than that of the cob. The phytosterol content of the kernel was higher in the order β- sitosterol 80.05% > campesterol 10.5% > stigmasterol 9.46%, but that of the cob was higher in the order β-sitosterol 59.43% > stigmasterol 31.72% > campesterol 10.98%. Based on these results, it appears that the phytosterols of the maize kernel are synthesized in the maize cob and are transferred to the kernel, because the precursors (Δ7-avenasterol, Δ7-stigmastenol, and Δ5- avenasterol) of major phytosterols were detected in maize cobs.

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키워드

cob identification kernel maize phytosterol quantification