The purpose of this study was to investigate the effects of endurance exercise on exercise strength on improving autophagy and insulin resistance in aging T2DM rats. T2DM was classified as type 2 diabetes induced when blood sugar was more than 300 mg/dl with high fat diet and streptozotocin(STZ; 40 mg/kg), with a total of 40, four groups: control group (n=10), low intensity group (n=10), moderate intensity group (n=10), and high intensity group (n=10). The training was conducted for 8 weeks (60 minutes/5 days/weeks) at a speed of 9-26 m/min and a slope of 10°, which is low intensity (40%), moderate intensity (60%) and high intensity (80%) of rodent VO2max. Protein was extracted from Gastrocnemius, and insulin-related factors (GLUT4, PGC-1α) and autophagy-related factors (Beclin-1, ATG7) were analyzed using Western blotting. The results showed significant increases in both insulin and autophagy-related factors in the exercise population, with the highest expression in the medium-intensity exercise group. Therefore, these results are thought to have a positive effect associated with cellular homeostasis by increasing the expression of autophagy along with blood sugar improvement as an effect of exercise on T2DM aging rat.