적색 양파에서 안토시아닌 함량 조절 양적형질유전자좌와 연관된 SNP 분자표지 개발

• 영문명: Development of Single Nucleotide Polymorphism Markers Linked to Quantitative Trait Loci Controlling Anthocyanin Content in Red Bulb Onion (Allium cepa L.)
• 발행기관: 한국육종학회
• 저자명: 류현욱(Hyunwook Ryu) 김성길(Sunggil Kim) 이혜은(Hye-Eun Lee) 한지원(JiWon Han) 이준대(Jundae Lee)
• 간행물 정보: 『한국육종학회지』Vol.53 No.2, 116~126쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2021.06.30

영문 초록

The bulb onion (Allium cepa L.), one of the most important vegetables worldwide, contains various functional compounds suchas quercetin, allicin, and flavonoids. Red onions are rich in anthocyanins, a flavonoid that is a functional phytochemical with antioxidativeand anticancer activities. In the previous study, two quantitative trait loci (QTLs) (qAC4.1 and qAC4.2) controlling the anthocyanin contentwere identified on chromosome 4 in an F2 population derived from a cross between A. cepa ‘SP3B’ and ‘H6’. In this study, we developedsingle nucleotide polymorphism-based high-resolution melting (HRM) markers linked to QTLs qAC4.1 and qAC4.2. In addition, we constructeda new genetic linkage map of chromosome 4 using HRM markers and performed a QTL analysis. The QTL qAC4.1 was false, while qAC4.2was a major QTL. The QTL peak position, logarithm of the odds value, and phenotypic variance explained of qAC4.2 was 53.6 cM, 7.45, and 22.51%,respectively. Four HRM markers (AC4.2_65336.1_1123-HRM, AC4.2_53230.3_454-HRM, AC4.2_11999.1_756-HRM, and AC4.2_14596.1_345-HRM)within the QTL region of qAC4.2 were developed in this study. The average anthocyanin content of B (homozygous paternal) genotypes washigher than that of A (homozygous maternal) and H (heterozygous) genotypes for all markers. Consequently, these markers will be useful formarker-assisted selection to develop onion cultivars with high anthocyanin content.

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anthocyanin bulb onion HRM marker QTL SNP