Regeneration from Storage Root Disk Culture of Purple Sweet Potato

• 발행기관: 한국자원식물학회
• 저자명: Hyejeong Park Hyeonyong Park
• 간행물 정보: 『한국자원식물학회지』제28권 제3호, 363~369쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.06.30

영문 초록

Sweet potato has low regeneration capacity, which is a serious obstacle for the fruitful production of transgenic plants. Simple and rapid regeneration method from storage root explants of purple sweet potato (Ipomoea batatas L.) was investigated. The embryogenic callus was observed from 4 cultivars and its highest rate was induced at 1 μM 2,4-D after 5 weeks of culture. Result revealed that a low concentration of 2,4-D and low light intensity was important factors for embryogenic callus formation. After subculture on medium with 5 μM ABA for 4 days, subsequently, occurred the regeneration of shoots within 4 weeks when these embryogenic callus was transferred onto the MS hormone free medium. Regenerated shoots were developed into platelets, and grown normal plants in the greenhouse. We developed a simple and quickly protocol to regenerate plantlets in storage root explants of purple sweet potato. This regeneration system will facilitate tissue culture and gene transfer research of purple sweet potato.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgment

References

키워드

Ipomoea batatas Embryogenic callus formation Somatic embryogenesis Plant Regeneration