약용자원식물 구절초의 고소득화를 위한 번식체계 확립 및 재분화 식물체의 광합성 능력증대 I. 구절초의 기내배양 및 재분화 식물체의 RAPD 분석

• 영문명: Study on the Propagation System and the Photosynthetic Rate of Chrysantemum zawadskii H.
• 발행기관: 한국자원식물학회
• 저자명: 김정률
• 간행물 정보: 『한국자원식물학회지』제11권 제1호, 1~8쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1998.02.28

영문 초록

This study was conducted to establish mass propagation system from the axillary bud culture of chrysanthemum zawadskii H. which was used as material of medicinal plants. Shoot egeneration was better on MS medium with NAA and BA. The optimum concentraions of growth regulator for shoot regeneration differed depending on accessionsof C. Zawadskii. Shoot regeneration in Keungucheolcho was better on MS Medium with NAA 0.01mg/1 and BA 0.1mg/1 while Hyangrobonggucheocho was better with NAA 0.1mg/1and BA 0.3mg/1. Addition of NAA into medium was effective for induction of root from shoots regenerated. Shoot multiplcation was more effective when 10mg/1 spermine was added into medium than when other polyamines were treated ino medium . Randomly and specifically amplified polymorphic DAC banding patterns based on polymerase chain reaction (PCR) analysis were used to assess the genetic variation of plants regenerated from in vitro culture.

키워드

axillary bud culture shoot regeneration RAPD analysis