Optimized Shoot Induction and Histological Study of in vitro Cultured Korean Soybean Cultivars

• 발행기관: 한국자원식물학회
• 저자명: Vipada Kantayos Chang-Hyu Bae
• 간행물 정보: 『한국자원식물학회지』제32권 제3호, 237~243쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.06.30

영문 초록

Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, ‘Dawon’, ‘Pungsan’, ‘Daewon’, ‘Taekwang’ and ‘Chongdoo 1’ by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, ‘Dawon’ and ‘Pungsan’. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 ㎎/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in ‘Dawon’ cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgement

References

키워드

Cotyledonary node Histological study in vitro culture Shoot induction Soybean cultivar