Molecular Characterization and Expression Analysis of Ascorbate Peroxidase in Codonopsis lanceolata (S. et Z.) Trautv

• 발행기관: 한국자원식물학회
• 저자명: In Jun-Gyo Lee Eun-Kyung Kim Ha-Na Yoon Jae-Ho Lee Mee-Sook Yang Deok-Chun
• 간행물 정보: 『Plant Resources』Vol.8 No.3, 194~201쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2005.12.31

영문 초록

A cytosolic ascorbate peroxidase, hydrogen peroxide-scavenging enzyme, was characterized from Codonopsis lanceolata. The cytosolic ascorbate peroxidase cDNA (CAPX) was 983 nucleotides long and possess an open reading frame of 753 bp with 251 amino acids (MW 27.9 kDa) with pI 5.61. The deduced amino acid sequence of CAPX shows high homology to other known cytosolic APXs (78~83%, but the CAPX was clustered independently from compared ten plant APXs. The CAPX gene was highly expressed in leaf and stem tissues, but not in root. When Codonopsis leaves cut using scalpel were soaked in 1 mM hydorgen peroxide, the expression of CAPX gene was suppressed.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

AKNOWLEDGEMENT

LITERATURE CITED

키워드

Ascorbate peroxidase CAPX Codonopsis lanceolata root RT-PCR