RAPD analyses using rapidly extracted genomic DNAs in plant species

• 발행기관: 한국육종학회
• 저자명: 이윤수(Youn Su Lee) 박철호(Cheol Ho Park) 강규영(Kyu Young Kang) 김남수(Nam Soo Kim)
• 간행물 정보: 『한국육종학회지』Vol.26 No.3, 287~293쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1994.09.30

영문 초록

The RAPD analysis with rapid genomic DNA extraction method in this study showed that the genotyping of plant species could be performed with relatively simple and economic method. The amount of extracted DNAs from a leaf disc was sufficient to carry out more than 100 PCR reactions, which make possible early diagnosis of the plant genotypes and thus reduce the time and labor to grow the plants until maturity. Cultivars in rice and soybean species were identified by the pattern of PCR amplified DNA fragments. RAPD analysis was also applied to check the genetic changes in somaclones of hare’s ear, from which many changes were observed among nineteen somaclones.

목차

ABSTRACT

Introduction

Materials and Methods

Results and Discussion

Concluding Remarks

摘要

Literature Cited

키워드

PCR RAPD Polymorphism Genome Genotype Cultivar Near isogenic line Somaclone