천궁(ligusticum chuangxiong) 현탁배양에서 체세포배의 발생과 식물체 재생

• 영문명: Development of somatic embryos and plant regeneration in suspension culture of ligusticum chuangxion horts
• 발행기관: 한국육종학회
• 저자명: 채영암(Young Am Chae) 박상언(Sang Un Park)
• 간행물 정보: 『한국육종학회지』Vol.26 No.2, 177~181쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1994.06.30

영문 초록

This study was carried out to investigate the possibility of plant regeneration through somatic embryogenesis in suspension culture of Ligusticum chuangxiong. Embryogenic callus was induced from cotyledon on MS medium. Callus growth and somatic embryo formation rate were higher in suspension culture when 2,4-D 0.5~1.0㎎/ℓ were added. MS medium was good for somatic embryo development and its higher rate was observed at half strength MS medium. Sucrose as carbon sources was the most effective and 1% sucrose was enough for somatic embryo formation in half strength MS medium. The rate of nitrate to ammonium nitrogen of 825㎎/ℓ : 238㎎/ℓ was appeared as proper level for maximum somatic embryo formation in suspension culture.

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키워드

Ligusticum chungxiong Somatic embryo Plant regeneration