Overcoming cross incompatibility between nicotiana tabacum L. and N. trigonophylla Dun. through in vitro fertilization

• 발행기관: 한국육종학회
• 저자명: Chang Sun Chung(鄭燦善)
• 간행물 정보: 『한국육종학회지』Vol.23 No.1, 30~34쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1991.03.30

영문 초록

Interspecific hybrids between Nicotiana tabacum L. (2n=48, ♀) and N. trigonophylla Dun. (2n=24, ♂) can not be obtained by conventional crossing because of the existance of strong sexual prefertilization barries. The technique of in vitro fertilization were applied to overcome this cross incompatibility. Ten placenta with intact ovules of N. tabacum were pollinated in vitro. by dusting pollen grains of N. trigonophylla. Finally, seven hybrid plants were obtained. All of the hybrid plants were morphological uniform and had 36 chromosomes which were additive sum of the haploid chromosomes of the parents. From the results, it is considered that the in vitro fertilization is useful for overcoming sexual prefertilization barriers.

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ABSTRACT

INTROUDUTION

MATERIALS AND METHODS

RESULTS

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摘要