Differentially Expressed Genes Related to Cold-resistance in Barley (Hordeum vulgare L. cy. Nagaoka)

• 발행기관: 한국육종학회
• 저자명: Jong Un Chun Jeong Seon Park Chang Hyu Bae Jeong Sheop Shin
• 간행물 정보: 『한국육종학회지』Vol.41 No.2, 73~83쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2009.06.30

영문 초록

To investigate genes related to vernalization and cold- resistance in barley (Hordeum vulgare L. cv. Nagaoka), differentially expressed genes were identified from cold-resistant barley leaves with suppression subtractive hybridization (SSH) and Northern blot analyses. The nucleotide and the deduced amino acid sequences of the putative gene products were compared. The bvrn-7 showed high homology(84%) with gene related to vernalization, and the bvrn-3, bvrn-12, bvrn-28, bvrn-29 and bvrn-36 related to cold-resistant genes had high identity of 88~98% with low temperature-induced genes. The results indicate that the 6 genes were closely related to vernalization and cold-resistance during low temperature treatment.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

키워드

suppression subtractive hybridization (SSH),barley,vernalization,cold-resistant gene