Karyotype Analyses of a rice Cultivar ‘Nakdong’ and its four Genetically Modified Events by Conventional Staining and Fluorescence in situ Hybridization

• 발행기관: 한국육종학회
• 저자명: Eun Jin Jeon Kwang Bok Ryu Hyun Hee Kim
• 간행물 정보: 『한국육종학회지』Vol.43 No.4, 173~180쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.08.30

영문 초록

Conventional staining and fluorescence in situ hybridization (FISH) karyotypes of the non-genetically modified (GM) parental rice line, ‘Nakdong’ (Oryza sativa L. japonica), and its four GM rice lines, LS28 (event LS30-32-20-1), Cry1Ac1 (event C7-1-9-1), and LS28 × Cry1Ac1 (events L/C1-1-3-1 and L/C1-3-1-1) were analyzed using 5S and 45S rDNAs as probes. Both parental and transgenic lines were diploids (2n=24) with one satellite chromosome pair. The lengths of the prometaphase chromosomes ranged from 1.50 to 6.30 µm. Four submetacentric and eight metacentric pairs comprised the karyotype of ‘Nakdong’ and its four GM lines. One pair of 5S rDNA signals was detected near the centromeric region of chromosome g in both the parental and transgenic lines. The 45S rDNA signals were detected on the secondary constrictions of the satellite chromosome pair in both the parental and transgenic lines. There was no significant difference in chromosome size, length, and composition between ‘Nakdong’ and its four GM lines. This research was conducted as a preliminary study for chromosomal detection of transgenes in GM rice lines and would be useful for their breeding programs.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

REFERENCES

키워드

Karyotype Genetically modified rice FISH Probe