SRAP을 이용한 국내육성 심비디움 품종의 유전적 다양성 분석

• 영문명: Analysis of Genetic Diversity in Cymbidium Varieties Using SRAP
• 발행기관: 한국육종학회
• 저자명: 박부희(Pue Hee Park) 김미선(Mi Seon Kim) 이영란(Young Ran Lee) 박필만(Pil Man Park) 이동수(Dong Soo Lee) 예병우(Byeong Woo Yae)
• 간행물 정보: 『한국육종학회지』Vol.43 No.5, 399~404쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.10.30

영문 초록

Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between F 1 varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.

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키워드

Cymbidium Breeding Dendrogram Diversity