국내 밀 품종의 글리아딘 및 Spa 유전자 평가

• 영문명: Evaluation of Gliadin and Storage Protein Activator (Spa) in Korean Wheat Cultivars
• 발행기관: 한국육종학회
• 저자명: 김지은(Ji-Eun Kim) 윤영미(Young-Mi Yoon) 조성우(Seong Woo Cho) 강천식(Chon-Sik Kang) 이종열(Jong-Yeol Lee) 박철수(Chul Soo Park)
• 간행물 정보: 『한국육종학회지』Vol.48 No.3, 206~216쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.09.30

영문 초록

Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.

목차

1. 서언

2. 재료 및 방법

3. 결과 및 고찰

4. 적요

5. 사사

키워드

Wheat Gliadin Storage Protein Activator (Spa) A-PAGE,Allelic variation