악하선 분비와 선혈류에 미치는 L-NAME와 Glyburide의 영향

• 영문명: Effects of L-Name and Glyburide on the salivary scrtion and vasodilation in the submandibular gland of the cat
• 발행기관: 대한구강악안면외과학회
• 저자명: 김기영(GI Young Kim) 김원재(Won Jae Kim) 류선열(Sun Youl Ryu)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제21권 제3호, 335~345쪽, 전체 11쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1995.07.30

영문 초록

The present study was designed to investigate whether and to what extent endothelium-derived nitric oxide (EDNO) and endothelium-derived hyperpolarizing factor (EDHF) are involed in vasodilation and salivation induced by parasympathetic stimulation. Effects of Nw-notro-L-arginine-methyl ester (L-NAME), which blocks the synthesis of EDNO from L-arginine, and glyburide, which blocks ATP-sensitive K+ channel (KATP), on vasodilation and salivation induced by chorda stimulation or administration of various vasodilators in the submandibular gland was examined in anesthetized cats. Effects of L-NAME and glyburide on K+ efflux induced by carbachol was also examined using the excised submaxillary slice. 1. Salivary secretion in response to the chorda stimulation (1-20 Hz) was completely blocked by treament with atropine (1 mg/kg). Increased blood flow response to the chorda stimulation was significantly attenuated when low frequency (1, 2, 5 Hz)-stimuli were applied, while that to a higher frequency (10, 20 Hz)-stimuli were not affected. 2. Intra-arterial infusion of L-NAME (100mg/kg) or glyburide (2 mg/kg) significantly diminished the vasodilatory and salivary responses to the chorda stimulation regardless of the stimuli frequencies used. 3. Intra-arterial infusion of L-NAME (100 mg/kg) or methylene blue (2 mg/kg) markedly diminished the vasodilatory responses to acetylcholine (5μg/kg), vasoactive intestinal peptide (5μg/kg) or bradykinin (5μg/kg). 4. The vasodilator responses to the ACh, VIP, or BK (5μg/kg) was significantly attenuated by intra-arterial infusion of glyburide (2 mg/kg). 5. In the excised submandibular slice, K+ efflux in response to carbarchol (10E-5mol/L) was significantly attenuated by pretreatment with glyburide (10E-5 mol/L) diminished, although transiently, the K+ efflux. 6. In the isolated submaxillary artery precontracted with phenylephrine (10E-5 mol/L), the vasorelaxation induced by ACh (10E-7 mol/L), was reversed by methylene blue and BaCl₂ (10E-6 mol/L). These results suggest that EDNO and EDHF are implicated in the parasympathetic vasodilation and salivary secretion in the submandibular gland.

목차

Abstract

I. 서론

II. 실험방법

III. 실험성적

IV. 고안

V. 결론

참고문헌

키워드

EDNO DEHF L-NAME Glyburide KATP