백서 설 발생과정에 작용하는 Cyclins, Cyclin Dependent Kinases, CDK Inhibitors의 변화

• 영문명: CHANGES OF CYCLINS, CYCLIN DEPENDENT KINASES, CYCLIN DEPENDENT KINASE INHIBITORS DURING GLOSSAL DEVELOPMENT IN THE RATS
• 발행기관: 대한구강악안면외과학회
• 저자명: 구명삼(Myoung Sam Koo) 진우정(Woo Jeong Jin)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제23권 제4호, 597~611쪽, 전체 15쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1997.10.30

영문 초록

The molecular mechanisms that regulate glossal muscle cell cycle and terminal differentiation remain largely unknown. To determine which cyclins, cyclin dependent kinases (CDKs), cyclin dependent kinase inhibitors (CKIs) are important for glossal cell proliferation, we have examined expression of cyclins CDKs, CKIs during normal glossal muscle development in the rat. All cyclins, CDKs, and KIP/CIP family of CKIs were highly expressed during fetal glossal muscle development, then they decreased at different rates after birth. While the mRNAs of cyclin D1, D3, E, A, and B decreased gradually m glossal muscle during all stages of development, the protein levels of these cyclins decreased differently in tongue during pre- and postnatal development. While the functionally active form of cyclin D1, cyclin D3 and E proteins were observed until 7 days after birth, cyclin A and B proteins were decreased more slowly. While the CDK4, CDK6, CDK2, cdc2, and proliferating cell nuclear antigen (PCNA) proteins were highly present during fetal glossal muscle development and gradually decreased during postnatal development. Particularly, cdc2 levels decreased markedly after birth. lmmunohistochcmical data for PCNA was consistent with Western blotting data for PCNA temporally and spatially. The mRNA and protein levels of p21, p27, and p57 were high, then their levels changed differently during glossal development. While the mRNA levels of p21 and p57 decreased gradually, the mRNA level of p27 did not change during glossal development. While the protein levels of p21 and p57 in tongue decreased markedly after birth, the protein levels of p27 increased slightly after birth, then decreased at adulthood. These findings suggest that the all cyclins and CDKs observed are involved in glossal muscle cell cycle, and reduction of cyclins and CDKs and induction of p21 are associated with the withdrawal of glossal muscle cell cycle after birth.

목차

Abstract

I. 서론

II. 실험재료 및 방법

III. 실험성적

IV. 총괄 및 고안

V. 결론

참고문헌