치성낭종 상피세포의 증식, 분화 및 세포능동사망현상에 관한 면역조직화학적 연구

• 영문명: AN IMMUNOHISTOCHEMICAL STUDY ON PROLIFERATION, DIFFERENTIATION, AND APOPTOSIS IN THE EPITHELIAL CELLS OF THE ODONTOGENIC CYSTS
• 발행기관: 대한구강악안면외과학회
• 저자명: 정성훈(Seong Hoon Chung) 박영욱(Young Wook Park) 주재용(Jae Yong Joo) 이석근(Suk Keun Lee)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제26권 제5호, 470~480쪽, 전체 11쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2000.10.30

영문 초록

The epithelium of odontogenic cyst seems to be in a specific status of cellular proliferation and cytodifferentiation. With the identification of various genes, which play essential roles in the specific stages of cellular proliferation and differentiation, the cellular conditions of odontogenic cyst epithelium need to be reevaluated. This study aimed to estimate the degree of proliferating, differentiating and apoptotic activities of odontogenic cyst epithelium using antisera of PCNA, Ki-67, MPM-2, transglutaminase C, heat shock protein 70 and ApopTag？？ method in 19 cases of odontogenic cysts. Cellular changes of the cyst epithelium were measured by intensity of each immunohistochemical staining. Results were as follows: 1. The proliferating activity of the cyst epithelium was slightly lower than that of normal oral mucosal epithelium, with the use of primary antibodies against PCNA, Ki-67, and MPM-2. And the proliferating activity of the epithelium in OKC group was even higher than that of the epithelium in non-OKC group. 2. The odontogenic cysts showed weakly positive reaction with transglutaminase C, but strongly positive reaction with HSP 70. 3. Occasionally, only a few apoptotic cell was observed in the superficial keratin layer of OKC. 4. The hyperplastic cyst epithelium infiltrated with mild inflammatory cells showed diffusely positive reaction with different proliferating factors. From the above results, we presumed that the endogenous proliferating and differentiating activity of the cyst epithelium was slightly lower than that of normal oral mucosal epithelium, and also supposed that the cyst epithelium could be reactivated for the further proliferation by the exogenous factors, such as inflammatory reaction and any chemicophysical irritations.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구재료 및 방법

Ⅲ. 연구결과

Ⅳ. 총괄 및 고찰

Ⅴ. 결론

참고문헌

키워드

Proliferation Differentiation Apoptosis