노물질의 세포독성 평가법으로 Colony Forming Efficiency Assay에 대한 검증연구

• 영문명: Pre-validation of Colony Forming Efficiency Assay for Assessing the Cytotoxicity of Nanomaterials
• 발행기관: 한국환경보건학회
• 저자명: 조은혜(Eunhye Jo) 이재우(Jaewoo Lee) 박선영(Sun-Young Park) 김필제(Pilje Kim) 최경희(Kyunghee Choi) 엄익춘(Igchun Eom)
• 간행물 정보: 『1. 한국환경보건학회지』제41권 제1호, 17~23쪽, 전체 7쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2015.02.28

영문 초록

Objectives: The cytotoxcities of Au, Ag, SWCNT, SiO 2 , and ZnO nanomaterials were evaluated in order to assess their potential toxicological effects in in vitro cell models using colony forming efficiency (CFE) assay. Methods: The CFE assay of the test materials was carried out on Hep G2 cells. The size distribution of nanomaterials was studied by transmission electron microscopy (TEM). Changes in cell viability after treatment with a toxicant will result in a decreased number of colonies formed in comparison to solvent. Results: The TEM images show that all the particles except SWCNT and ZnO can be considered approximately spherical. The gold and SiO 2 nanoparticles show no response (no toxicity) in concentration response experiments. A statistically significant toxic effect was found in Hep G2 cells treated with Ag, SWCNT and ZnO nanomaterials. Conclusion: In this study, we considered CFE assay to be a promising test for screening studies for cytotoxicity with physicochemical analysis.

목차

I. 서 론

II. 연구내용 및 방법

III. 결 과

IV. 고 찰

키워드

Colony forming efficiency assay Cytotoxicity Nanomaterials