소변 중 다환방향족탄화수소 대사체의 분석법 확립 및 교차분석

• 영문명: Method Development and Cross Validation of Analysis of Hydroxylated Polycyclic Aromatic Hydrocarbons (OH-PAHs) in Human Urine
• 발행기관: 한국환경보건학회
• 저자명: 박나연(Na-Youn Park) 전중대(Jung-Dae Jeon) 구혜령(Hyeryeong Koo) 김정환(Jung Hoan Kim) 이은희(Eun-Hee Lee) 이경무(Kyungmu Lee) 문철진(Cheoljin Mun) 고영림(Younglim Kho)
• 간행물 정보: 『1. 한국환경보건학회지』제41권 제5호, 358~367쪽, 전체 10쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2015.10.30

영문 초록

Objectives: This study was performed to evaluate the analytical method for PAH metabolites in human urine using enzyme hydrolysis and solid-phase extraction coupled with LC-(ESI)-MS/MS technique. Methods: We employed HPLC tandem mass spectrometry techniques with appropriate pre-treatment for analysis of 16 OH-PAHs in human urine. Samples were hydrolysis by β-flucuronidase/Aryl sulfatase, and target compounds were extracted by solid-phase extraction with a strata-x cartridge. Cross-validation was performed between Eulji University and Green Cross laboratories with 200 human urine samples. Results: The accuracies were between 90.3% and 118.8%, and precisions (relative standard deviations) were lower than 10%. The linearity obtained was satisfying for the 16 OH-PAH compounds, with a coefficient of determination (r 2 ) higher than 0.99. The results of cross-validation at the two organizations were compared by ICC (interclass correlation coefficient) values. The cross-validation results were excellent or good for all compounds. Conclusion: An analytical method was validated for low nanogram levels of 16 OH-PAHs in human urine. Also, satisfying results were obtained for method validation such as accuracy, precision and ICC of cross-validation.

목차

I. 서 론

II. 재료 및 방법

III. 연구결과

IV. 고 찰

V. 결 론

키워드

Cross validation LC-MS/MS OH-PAHs urine