체외 배양된 SNU-1 세포주에서 transglutaminase C antisense inhibition이 일으키는 세포핵질 변화

• 영문명: NUCLEAR MATRIX CHANGES BY THE ANTISENSE INHIBITION OF TRANSGLUTAMINASE C IN IN VITRO CULTURE OF SNU-1 CELLS
• 발행기관: 대한구강악안면외과학회
• 저자명: 장재현(Jae-Hyun Jang) 이석근(Suk-Keun Lee) 박영욱(Young-Wook Park)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제29권 제2호, 86~94쪽, 전체 9쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2003.04.30

영문 초록

It has been known that transglutaminase C (TGase C, TGase II) is directly participated in the DNA organization of chromosome, and affects the cellular processes such as proliferation, differentiation, and apoptosis of cells, but still not known what mechanism is working on. In this study, the cytogenetic and the immunohistochemical methods were used to observe the TGase C expression in the nuclear chromosome of the proliferating cells, especially in mitotic stage. The human gastric adenocarcinoma (SNU-1) cell line was used for immunohistochemistry and antisense inhibition study in vitro. The present study was also aimed to disclose the efficiency of antisense inhibition by using antisense oligonucleotide DNA labeled with fluorescence, and found that anti-TGase C probe was diffusely infiltrated into the cytoplasm and the nucleus of the cell. By the antisense inhibition the nuclei of SNU-1 cells became rough nuclear shape, as they were greatly reduced in TGase C immunoreactivity both for the normal and apoptotic SNU-1 cells. However, it is clearly presumed that the TGase C directly interacts with the chromosome of SNU-1 cells and it may play an important role in the division and organization of the chromosome during the mitotic stage.

키워드

Transglutaminase C Antisense inhibition