제대혈 내피기원세포 및 간엽줄기세포의 분화에 대한 연구

• 영문명: ENDOTHELIAL PROGENITOR CELLS AND MESENCHYMAL STEM CELLS FROM HUMAN CORD BLOOD
• 발행기관: 대한구강악안면외과학회
• 저자명: 김은석(Eun-Seok Kim) 김현옥(Hyun-Ok Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제31권 제1호, 39~45쪽, 전체 7쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2005.02.28

영문 초록

Stem cell therapy using mesenchymal stem cells(MSCs) transplantation have been paid attention because of their powerful proliferation and pluripotent differentiating ability. Although umbilical cord blood (UCB) is well known to be a rich source of hematopoietic stem cells with practical and ethical advantages, the presence of mesenchymal stem cells (MSCs) in UCB has been controversial and it remains to be validated. In this study, we examine the presence of MSCs in UCB harvests and the prevalence of them is compared to that of endothelial progenitor cells. For this, CD34+ and CD34- cells were isolated and cultured under the endothelial cell growth medium and mesenchymal stem cell growth medium respectively. The present study showed that ESC-like cells could be isolated and expanded from preterm UCBs but were not acquired efficiently from full-terms. They expressed CD14-, CD34-, CD45-, CD29+, CD44+, CD105+ cell surface marker and could differentiate into adipogenic and osteogenic lineages. Our results suggest that MSCs are fewer in full-term UCB compared to endothelial progenitor cells.

키워드

Mesenchymal stem cell Endothelial progenitor cell Umbilical cord Blood