설편평상피암에 있어서의 고밀도 SNP Genotyping 어레이를 이용한 전게놈북제수와 헤테로접합성 소실의 분석

• 영문명: Analysis of copy number abnormality (CNA) and loss of heterozygosity (LOH) in the whole genome using single nucleotide polymorphism (SNP) genotyping arrays in tongue squamous cell carcinoma
• 발행기관: 대한구강악안면외과학회
• 저자명: 黑岩 司(Kuroiwa Tsukasa) 山本信治(Yamamoto Nobuharu) 恩田健志(Onda Takeshi) 別所央城(Bessyo Hiroki) 藥師寺 孝( Yakushiji Takashi) 片倉 朗(Katakura Akira) 野伸夫(Takano Nobuo) 柴原孝彦(Shibahara Takahiko)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제37권 제6호, 550~555쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2011.12.30

영문 초록

Chromosomal loss of heterozygosity (LOH) is a common mechanism for the inactivation of tumor suppressor genes in human epithelial cancers. LOH patterns can be generated through allelotyping using polymorphic microsatellite markers; however, owing to the limited number of available microsatellite markers and the requirement for large amounts of DNA, only a modest number of microsatellite markers can be screened. Hybridization to single nucleotide polymorphism (SNP) arrays using Affymetarix GeneChip Mapping 10 K 2.0 Array is an efficient method to detect genome-wide cancer LOH. We determined the presence of LOH in oral SCCs using these arrays. DNA was extracted from tissue samples obtained from 10 patients with tongue SCCs who presented at the Hospital of Tokyo Dental College. We examined the presence of LOH in 3 of the 10 patients using these arrays. At the locus that had LOH, we examined the presence of LOH using microsatellite markers. LOH analysis using Affymetarix GeneChip Mapping 10K Array showed LOH in all patients at the 1q31.1. The LOH regions were detected and demarcated by the copy number 1 with the series of three SNP probes. LOH analysis of 1q31.1 using microsatellite markers (D1S1189, D1S2151, D1S2595) showed LOH in all 10 patients (100). Our data may suggest that a putative tumor suppressor gene is located at the 1q31.1 region. Inactivation of such a gene may play a role in tongue tumorigenesis.

목차

I. 제 언

II. 대상증례 및 연구방법

III. 결 과

IV. 고 찰

V. 결 어

키워드

Tongue squamous cell carcinoma Loss of heterozygosity Copy number abnormality Single nucleotide polymorphism tumor suppressor gene