수종의 항혈전제의 국소 세척이 미세혈관문합의 동맥 치유 및 개존에 미치는 영향

• 영문명: EFFECT OF ARTERIAL REPAIR AND PATENCY AFTER MICROVASCULAR ANASTOMOSIS WITH TOPICAL IRRIGATION OF VARIOUS ANTI-THROMBUS DRUGS
• 발행기관: 대한구강악안면외과학회
• 저자명: 최용철(Yong-Chul Choi) 김경욱(Kyung-Wook Kim) 김철환(Chul-Hwan Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제32권 제2호, 117~128쪽, 전체 12쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2006.04.30

영문 초록

Free flap transplantation with microvascular anastomosis has been successfully performed by development of surgical technique, materials and postoperative monitoring equipments of flap. But success rate of microvascular anastomosis is influenced by various factors, and failure rate is about 5-10%. The most influential factor for success rate is surgical technique and other factors that influence failure of microvascular anastomosis are ischemic time of free flap, thrombus formation of anastomosis region and vascular spasm. Many studies has been published in microvascular anastomosis with histologic effect for irrigating solution. But local irrigation solution has been used clinically in microvascular anastomosis, the comparison with each solution, microhistological study for endothelial cell repair and vascular patency has not been reported. The heparin which is anti-thrombotic agent, and urokinase which is fibrinolytic agent are used for this study. Vascular patency and thrombus formation in experimental micro-arterial anastomosis, and endothelial repair were observed with histologic analysis, scanning electron microscopy, transmission electron microscopic examination. The results were obtained as follows: 1. In vascular patency test in 30 minute and 7 days after micro-arterial anastomosis, equal effects of good vascular patency were obtained in group of local irrigation with heparin and urokinase. 2. In thrombus formation in 7 days after micro-arterial anastomosis, equal effects of minimal thrombus formation were obtained in group of local irrigation with heparin and urokinase. 3. In toluidin blue staining in 7 days after micro-arterial anastomosis, local destruction of endothelial cell and inner elastic lamina were seen and endothelial repair was not seen. 4. In scanning electron microscope examination in 7 days after micro-arterial anastomosis, endothelial cell was not seen in peripheral to suture materials, thrombus associated fibrin network was observed. 5. In transmission electron microscope examination in 7 days after microarterial anastomosis, inflammatory cell was seen within smooth muscle cells in site of endothelial cell destruction, smooth muscle cell around suture material were arranged irregularly, some collagenous change were seen. From the results obtained in this study, same results of good vascular patency and anti-thrombotic effect of heparin and urokinase were obtained as a local irrigation solution, and repair of endothelial cell was not seen in 7 days after micro-arterial anastomosis.

키워드

Microvascular Anastomosis Anti-thrombus drug Heparin Urokinase