두경부 편평상피세포암 세포주의 수립 및 특성

• 영문명: Establishment and Characterization of Cell Lines Derived from Squamous Cell Carcinoma of the Head and Neck
• 발행기관: 대한두경부종양학회
• 저자명: 김광현(Kwang Hyun Kim) 정필상(Phil-Sang Chung) 박현민(Hyun Min Park) 이재서(Chae Seo Rhee) 박재갑(Jae Gahb Park)
• 간행물 정보: 『대한두경부종양학회지』제12권 제2호, 181~187쪽, 전체 7쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 1996.11.30

영문 초록

We have characterized 4 human squamous carcinoma cell lines established from the larynx and hypopharynx area. All the cell lines were cultured in RPMI-1640 medium. During the growth they showed monolayer adherence pattern in culture flask. They showed tonofilament on transmission electromicroscopy which is characteristic of squamous cell epithelium. DNA finger-printing using Hinf-l proved them to be originated from different beings. Flow cytometric analysis revealed them to show aneuploidy. Immunohistochemical staining for cytokeratin was done using CK1, CK8.13, CK19 and CAM5.2 antibody, and produced various patterns of positivity. All the cell lines showed varying degrees of tumorigenecity in athymic nude mice when injected subcutaneously, but only heterotransplanted SNU-1041 cell line showed continuous tumor growth. Histopathologic findings of the heterotransplanted tumors were identical to those of the original tumors of patients. This study suggests that establishment of many different squamous cell lines might bestow great capability in researches of the head and neck cancer.

목차

Abstract

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연구재료 및 방법

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고찰

결론

References

키워드

Squamous cell carcinoma of the head and neck Cell lines Characterization