두경부 편평세포암종의 발암 원인으로 인간유두종 바이러스(Human Papilloma Virus)의 역할 및 이와 관련된 발암 기전에 관한 연구

• 영문명: The Etiologic Roles and Carcinogenic Mechanisms of Human Papilloma Virus in Head and Neck Squamous Cell Carcinoma
• 발행기관: 대한두경부종양학회
• 저자명: 신동현(Dong Hyun Shin) 이세영(Sei Young Lee) 구본석(Bon Seok Koo) 김세헌(Se-Heon Kim)
• 간행물 정보: 『대한두경부종양학회지』제25권 제1호, 28~32쪽, 전체 5쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 2009.05.30

영문 초록

Background ：The most frequently reported risk factors for head and neck suamous cell carcinoma are smo-king and alcohol. But in a recent overview, human papilloma virus(HPV) infection was revealed the important carcinogenic factor in oropharyngeal cancer. We aimed to clarify whether HPV directly effects on the oncoge-nesis and biologic behavior of hean and neck squamous cell carcinoma by comparison with infection preva-lence, and physical status of virus. Material and Method ：We used HPV genotyping DNA chip(Biocore, Korea, Seoul) arrayed by multiple oli-gonucleotide probes of L1 sequence of 26 types of HPV and HPV genotypes are identified by fluorescence scanner. The copy numbers of HPV E2 and E6 open reading frames(ORF) were assessed using a TaqMan-based 5’-exonu-clease quantitative real-time PCR assay. The ratio of E2 to E6 copy numbers was calculated to determine the physical status of HPV-16 viral gene. Results ：We observed a significant difference in HPV prevalence between tonsillar cancer group and con-trol group(73.1% vs. 11.6%), and most of the HPVs were type 16(87.2%) and integrated(94.1%) state. In terms of oral tongue cancer, we demonstrate that 30.5% has integrated HPV-16 in cancer tissue. But Glottic cancer only 1% is related to HPV-16 integration. Conclusion ：This study revealed significant relationship of HPV prevalence with oropharyngeal and oral tongue squamous cell carcinoma. Most of HPV were 16 type and integrated or mixed, HPV-16 integration could be directly related to the carcinogenesis.

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Human papillomavirus Integration Head and neck squamous cell carcinoma 인간유두종 바이러스 두경부 편평세포암종.