제품화된 무방부제 힐론 점안제가 각막상피세포에 미치는 영향

• 영문명: Effect of Preservative-Free Healon Eye Drop on Human Corneal Epithelial Cell in Vitro
• 발행기관: 대한안과학회
• 저자명: 김호윤 박영민 이종수,Ho Yun Kim, MD, Young Min Park, MD, Jong Soo Lee, MD, PhD,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume55,number11, 1698~1705쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.11.15

영문 초록

Purpose: To investigate the biologic effects of preservative-free artificial tear drops on cultured human corneal epithelial cells in vitro. Methods: The effects of preservative-free artificial tear drops (sodium hyaluronate 0.1% (Kynex^{Ⓡ, Alcon, Seoul, Korea), sodium hyaluronate 0.18% (Kynex2Ⓡ, Alcon, Seoul, Korea), and sodium hyaluronate 0.3% (Hyaluni eye drops 0.3%Ⓡ, Taejoon, Seoul, Korea)) on human corneal epithelial cells were evaluated. Cellular proliferation was determined using MTT (3- (4,5-dimethylthiazol- 2yl)-2,5-diphenyl-2H-tetrazolium bromide) and Ki-67 assays. Cellular migration was determined using CD44 and scratch wound assays. Cell damage was determined using the lactate dehydrogenase (LDH) assay and cellular morphologies using electronic microscopy and inverted microscopy. Results: Proliferation of corneal epithelial cells, as determined by the MTT and Ki-67 assays, was not significantly different between the different eye drops (p > 0.05). The measured value of cellular migration after exposure of the sodium hyaluronate 0.3%, as determined by mean CD44 percentage and scratch wound assay, was higher than that of the sodium hyaluronate 0.1% and sodium hyaluronate 0.18%, but the CD44 value was not significantly different (p > 0.05). The LDH titer tended to increase as the concentration of sodium hyaluronate increased (p > 0.05), but influence on cytoplasm, as determined by electronic microscopy, was not different. Conclusions: Among 3 preservative-free artificial tear drops, sodium hyaluronate 0.3% demonstrated increased migration of corneal epithelial cells. As the concentration of sodium hyaluronate in eye drops increased, the corneal cytotoxicity of corneal epithelial cells also increased. However, there was no significant difference among the 3 tear drops. J Korean Ophthalmol Soc 2014;55(11):1698-1705}

키워드

Artificial tear, Cornea, Epithelial cell, Hyaluronic acid