타액 중 Δ9-Tetrahydrocannabinol 및 11-Nor-9-carboxy-Δ9-Tetrahydrocannabinol의 분석법 확립 및 안정성 검토

• 영문명: Development of Quantification Method and Stability of Δ9-Tetrahydrocannabinol and 11-Nor-9-carboxy-Δ9-Tetrahydrocannabinol in Oral Fluid
• 발행기관: 대한약학회
• 저자명: 최혜영(Hyeyoung Choi) 백승경(Seungkyung Baeck) 장문희(Moonhee Jang) 최화경(Hwakyung Choi) 정희선(Heesun Chung)
• 간행물 정보: 『약학회지』제54권 제4호 (2010년), 226~231쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.08.31

영문 초록

Oral fluid has become increasingly popular as an alternative specimen in the field of driving under the influence of drugs (DUID) and work place drug testing. In this study, an analytical method for the detection and quantification of Δ9-tetrahydrocannabinol (THC) and its metabolite, 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) in oral fluid by SPE and GC-MS was established and fully validated. The stability of THC and THC-COOH in oral fluid during storage was also determined by examining the THC and THC-COOH concentration changes depending on time and container materials. Oral fluid samples were kept over 21 days at room temperature, -4oC and -20oC in two different specimen collection tubes; glass and polypropylene tubes. Three replicates for each condition with different temperature and types of a container were analyzed at five different time points over 21 days. When oral fluid samples were stored in glass tubes, the loss of both THC and THC-COOH was less than 10% at all room temperature, -4oC and -20oC. However, in polypropylene tubes, the loss of both THC and THC-COOH increased significantly over the study period. In particular, the concentration of THC decreased more rapidly than that of THC-COOH at room temperature and the maximal percentage of THC lost was 90.3% after 21 days. The result indicates that it would be necessary to collect oral fluid samples in glass containers and cool the samples until analysis in order to prevent the degradation of analytes.

키워드

oral fluid stability driving under the influence of drugs(DUID) cannabis THC solid phase extraction(SPE) GC-MS